6TPC

Crystal structure of Endoglucanase N194A from Penicillium verruculosum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2019-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 2 21 21
Unit cell lengths	80.372, 82.551, 90.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.652 - 1.522
R-factor	0.177624471113
Rwork	0.175
R-free	0.22185
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i6s
RMSD bond length	0.005
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.652	1.610
High resolution limit [Å]	1.522	1.522
Number of reflections	90739	14123
<I/σ(I)>	8.24
Completeness [%]	97.4
Redundancy	7.56
CC(1/2)	0.996	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	PEG 6000, Bis-Tris-Propan,20% PEG 6000, MPD, tert-butano