6TP9
c-type cytochrome NirC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.017, 81.366, 198.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.310 - 2.190 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.24670 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (dev-3707) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.160 | 2.469 |
| High resolution limit [Å] | 2.188 | 2.188 |
| Rpim | 0.058 | 0.416 |
| Number of reflections | 35413 | 1772 |
| <I/σ(I)> | 10.9 | 1.8 |
| Completeness [%] | 92.7 | |
| Redundancy | 13 | |
| CC(1/2) | 0.998 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 15.3%(v/v) Glycerol 100mM MES pH6.3 10.8% (w/v) PEG20000 |
