6TP0
Crystal structure of Bacillus paralicheniformis alpha-amylase in complex with maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 82.960, 82.960, 187.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.890 - 2.040 |
| R-factor | 0.1573 |
| Rwork | 0.156 |
| R-free | 0.18490 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6toy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.890 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.157 | 0.671 |
| Rmeas | 0.168 | 0.720 |
| Rpim | 0.058 | 0.257 |
| Number of reflections | 42384 | 2385 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 98.2 | 76.3 |
| Redundancy | 8.1 | 7.5 |
| CC(1/2) | 0.995 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 42-46 % Tacsimate, 10 mM CaCl2 |
