6TOU
Rabies virus glycoprotein PH domain in complex with the scFv fragment of broadly neutralizing human antibody RVC20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-01 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.945, 81.945, 155.927 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.506 - 2.587 |
R-factor | 0.1932 |
Rwork | 0.190 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Phyre2 homology model of RVC20 scFv |
RMSD bond length | 0.003 |
RMSD bond angle | 0.607 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.506 | 2.720 |
High resolution limit [Å] | 2.587 | 2.587 |
Rmeas | 0.106 | 3.758 |
Rpim | 0.021 | 0.705 |
Number of reflections | 15457 | 774 |
<I/σ(I)> | 21.374 | |
Completeness [%] | 89.5 | 33.3 |
Redundancy | 25.7 | 28.1 |
CC(1/2) | 0.999 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.7 uL of 18 mg/mL complex in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.7 uL of reservoir solution containing 100 mM Tris-Cl pH 8.0, 300 mM CaCl2, 22% w/v PEG4000 |