6TOR
human O-phosphoethanolamine phospho-lyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 137.998, 137.998, 121.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 69.090 - 2.050 |
R-factor | 0.165 |
Rwork | 0.163 |
R-free | 0.19890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g4i |
RMSD bond length | 0.010 |
RMSD bond angle | 1.605 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.090 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.102 | |
Rpim | 0.068 | |
Number of reflections | 54318 | 54318 |
<I/σ(I)> | 10.1 | |
Completeness [%] | 100.0 | |
Redundancy | 4.9 | |
CC(1/2) | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG 3350, 100 mM Bis Tris propane pH 7.0, 200 mM ammonium sulfate, and 3% MPD. |