6TOC
Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis (crystal form 3).
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000040 |
| Spacegroup name | P 42 |
| Unit cell lengths | 31.523, 31.523, 81.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.500 - 1.853 |
| Rwork | 0.209 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6to5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.523 | 1.970 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmeas | 0.073 | 2.760 |
| Number of reflections | 6769 | 1072 |
| <I/σ(I)> | 21.4 | 1 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 13.5 | |
| CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Bis-Tri pH 6.5, 0.1 M NaCl, 1.5 M (NH4)2SO4 |
