6TO9
Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis (crystal form 2)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.050, 70.050, 80.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.380 - 2.450 |
| R-factor | 0.2304 |
| Rwork | 0.228 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6to5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.698 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.380 | 2.590 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmeas | 0.164 | 2.770 |
| Number of reflections | 8761 | 1362 |
| <I/σ(I)> | 15.9 | 1.07 |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 19 | 18.4 |
| CC(1/2) | 1.000 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 0.1 M phosphate citrate pH 4.2, 0.2 M NaCl, 20% (w/v) PEG 8000 |
