6TO5
Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-19 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 70.400, 70.400, 148.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.170 - 2.380 |
R-factor | 0.2153 |
Rwork | 0.214 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iit |
RMSD bond length | 0.004 |
RMSD bond angle | 0.600 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.180 | 2.520 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmeas | 0.189 | 2.210 |
Number of reflections | 16576 | 2262 |
<I/σ(I)> | 14.9 | 1.48 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 20.8 | |
CC(1/2) | 1.000 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 0.1 M phosphate citrate pH 4.2, 0.2 M NaCl, 20% (w/v) PEG 8000 |