6TNO
Crystal structure of the human Arc N-lobe bound to stargazin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.390, 29.770, 71.790 |
| Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.182 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x3h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.048 | 0.451 |
| Number of reflections | 17135 | 1246 |
| <I/σ(I)> | 18.6 | 2.9 |
| Completeness [%] | 97.8 | 97.9 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.999 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 1.4 M trisodium citrate |
