6TNE
Crystal structure of receiver domain from Hybrid Histidine Kinase CckA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 37.430, 42.900, 65.037 |
| Unit cell angles | 90.00, 94.36, 90.00 |
Refinement procedure
| Resolution | 28.158 - 1.250 |
| R-factor | 0.1656 |
| Rwork | 0.165 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Lpd1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.484 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP (11.7.01) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.780 | 35.780 | 1.270 |
| High resolution limit [Å] | 1.250 | 6.720 | 1.250 |
| Rmerge | 0.046 | 0.038 | 0.379 |
| Rmeas | 0.050 | 0.042 | 0.412 |
| Rpim | 0.019 | 0.017 | 0.161 |
| Total number of observations | 184150 | 1058 | 9508 |
| Number of reflections | 28291 | 186 | 1469 |
| <I/σ(I)> | 16.9 | 36.3 | 4.7 |
| Completeness [%] | 98.5 | 90.2 | 99.8 |
| Redundancy | 6.5 | 5.7 | 6.5 |
| CC(1/2) | 0.999 | 0.999 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.15 M Potassium thiocyanate, 0.1 M Sodium cacodylate pH 6.5, 20% v/v PEG 600 |
