6TMU
Crystal structure of the chaperonin gp146 from the bacteriophage EL 2 (Pseudomonas aeruginosa) in presence of ATP-BeFx, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 145.516, 151.461, 261.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.540 |
| R-factor | 0.2516 |
| Rwork | 0.250 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EMD-6492 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS (VERSION Jan 26, 2018) |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | MOLREP (11.4.06) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.470 | 49.470 | 3.620 |
| High resolution limit [Å] | 3.540 | 16.980 | 3.540 |
| Rmerge | 0.111 | 0.056 | 1.119 |
| Rmeas | 0.125 | 0.065 | 1.255 |
| Rpim | 0.056 | 0.032 | 0.557 |
| Total number of observations | 348403 | 2571 | 21929 |
| Number of reflections | 70880 | 692 | 4516 |
| <I/σ(I)> | 7.9 | 22.3 | 1.3 |
| Completeness [%] | 99.8 | 94.7 | 99.9 |
| Redundancy | 4.9 | 3.7 | 4.9 |
| CC(1/2) | 0.997 | 0.994 | 0.465 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 8 % PEG-6000, 0.15 M NaCl and 0.1 M Tris-HCl pH 8.0 |
