6TMM

BIL2 domain from T.thermophila BUBL1 locus (C1A-N143A)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	133.477, 72.769, 70.264
Unit cell angles	90.00, 97.87, 90.00

Refinement procedure

Resolution	69.602 - 2.398
Rwork	0.198
R-free	0.26650
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.287
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.602	2.540
High resolution limit [Å]	2.398	2.398
Rmerge	0.060	0.593
Rmeas	0.071	0.704
Number of reflections	26317	8236
<I/σ(I)>	12.25	1.78
Completeness [%]	98.9	98.9
Redundancy	3.36	3.37
CC(1/2)	0.998	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	Mg formate 0.1 M, PEG3350 19%, Tris 50 mM, NaCl 250 mM.