6TM8

Crystal structure of glycoprotein D of Equine Herpesvirus Type 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	73.095, 59.554, 69.675
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.170 - 1.900
R-factor	0.1766
Rwork	0.175
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sqj
RMSD bond length	0.012
RMSD bond angle	1.048
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.900	1.900
Rmeas	0.174	1.268
Number of reflections	23671	10835
<I/σ(I)>	8.96
Completeness [%]	99.5	78.2
Redundancy	5.9	3.5
CC(1/2)	0.995	0.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM MgCl2, 100 mM MES pH 6.5, 30% (w/v) PEG 400