6TM6
MUC2 CysD1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-10-03 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.879, 57.854, 27.471 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.230 - 1.630 |
R-factor | 0.1774 |
Rwork | 0.174 |
R-free | 0.21120 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.961 |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.460 | 1.689 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 10882 | 737 |
<I/σ(I)> | 37.79 | |
Completeness [%] | 94.9 | |
Redundancy | 22.9 | |
CC(1/2) | 1.000 | 0.938 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM citrate/phosphate buffer, pH 4.4, with 15% ethanol and 1% PEG 1K |