6TM0

N-Domain P40/P90 Mycoplasma pneumoniae complexed with 6'SL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	116.331, 107.380, 160.451
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	80.220 - 2.800
R-factor	0.211
Rwork	0.208
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rj1
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	autoXDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.220	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	47184	6819
<I/σ(I)>	7.4
Completeness [%]	97.0
Redundancy	6.6
CC(1/2)	0.996	0.252

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG3350 and MetOH