6TM0
N-Domain P40/P90 Mycoplasma pneumoniae complexed with 6'SL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.331, 107.380, 160.451 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 80.220 - 2.800 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rj1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.220 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 47184 | 6819 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 97.0 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.996 | 0.252 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG3350 and MetOH |
