6TLY

Crystal structure of the unconventional kinetochore protein Bodo saltans KKT2 central domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.28297
Spacegroup name	I 2 2 2
Unit cell lengths	38.961, 53.512, 83.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.800
R-factor	0.1963
Rwork	0.195
R-free	0.22490
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.020	45.040	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.102	0.072	0.520
Rpim	0.050	0.035	0.330
Number of reflections	8158	459	334
<I/σ(I)>	8.7
Completeness [%]	97.1	97.9	77.9
Redundancy	4.6	4.2	2.8
CC(1/2)	0.990	0.990	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	40% PEG 400, 0.2 mM (NH4)2SO4, 100 mM Tris-HCL pH 8