6TLX
Crystal structure of the unconventional kinetochore protein Perkinsela sp. KKT2a central domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 64 |
Unit cell lengths | 113.845, 113.845, 46.013 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.000 - 2.870 |
R-factor | 0.2538 |
Rwork | 0.253 |
R-free | 0.27480 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.080 |
Data reduction software | DIALS (1.8.4) |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.920 | 56.930 | 2.920 |
High resolution limit [Å] | 2.870 | 7.770 | 2.870 |
Rmerge | 0.102 | 0.048 | 1.045 |
Rmeas | 0.105 | ||
Rpim | 0.024 | 0.240 | |
Number of reflections | 7986 | 432 | 357 |
<I/σ(I)> | 16.4 | 60.5 | 2.1 |
Completeness [%] | 99.7 | 100 | 91.8 |
Redundancy | 19.2 | 16.1 | 19.5 |
CC(1/2) | 1.000 | 0.990 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 19% MPD, 50 mM Hepes, pH 7.5, 10 mM MgCl2 |