6TLI
THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.120, 94.120, 130.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| Rwork | 0.168 |
| R-free | 0.22200 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.000 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.126 | 0.404 |
| Number of reflections | 20404 | |
| <I/σ(I)> | 5.2 | 1.9 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | TLN | 4.6 (mM) | |
| 2 | 1 | 1 | DMSO | 45 (%) | |
| 3 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 4 | 1 | 1 | 2.5 (M) |
