6TKU
Crystal structure of a capsule-specific depolymerase produced by Klebsiella phage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97590 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 71.726, 71.726, 208.197 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.910 - 1.800 |
| R-factor | 0.2069 |
| Rwork | 0.204 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.686 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.232 |
| Number of reflections | 59968 | 4226 |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium citrate tribasic pH 7.0 and 20% w/v polyethylene glycol 3,350. |
