6TJY
Crystal structure of haemagglutinin from (A/seal/Germany/1/2014) seal H10N7 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.421, 212.932, 156.901 |
Unit cell angles | 90.00, 101.51, 90.00 |
Refinement procedure
Resolution | 35.040 - 2.820 |
R-factor | 0.2181 |
Rwork | 0.216 |
R-free | 0.25670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d00 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.048 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.040 | 2.870 |
High resolution limit [Å] | 2.820 | 2.820 |
Number of reflections | 105212 | 5226 |
<I/σ(I)> | 9.7 | |
Completeness [%] | 98.2 | |
Redundancy | 2.8 | |
CC(1/2) | 0.995 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2%-4% PEG6000, 0.1M HEPES pH7.0 |