6TJW

Crystal structure of the haemagglutinin mutant (Gln226Leu, Del228) from an H10N7 seal influenza virus isolated in Germany

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	69.290, 210.460, 82.751
Unit cell angles	90.00, 101.40, 90.00

Refinement procedure

Resolution	41.600 - 2.310
R-factor	0.2409
Rwork	0.239
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d00
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.090	2.430
High resolution limit [Å]	2.310	2.310
Number of reflections	101054	14698
<I/σ(I)>	5.5
Completeness [%]	99.8
Redundancy	3.8
CC(1/2)	0.985	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	10% PEG6000, 0.1M bicine pH9.0