6TJP

Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve - P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-05
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	119.850, 238.570, 265.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.470 - 3.740
R-factor	0.246
Rwork	0.244
R-free	0.28570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Partial cryo-EM model
RMSD bond length	0.003
RMSD bond angle	0.685
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.470	3.870
High resolution limit [Å]	3.740	3.740
Number of reflections	78414	7216
<I/σ(I)>	9.8
Completeness [%]	88.6
Redundancy	3.5
CC(1/2)	0.996	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M CaCl2, 0.1 M HEPES pH 7.5 and 18 % [w/v] PEG 400