6TJ5

T. gondii myosin A trimeric complex with ELC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 41
Unit cell lengths	87.317, 87.317, 56.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.580 - 2.394
R-factor	0.1915
Rwork	0.189
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgh
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.580	2.480
High resolution limit [Å]	2.394	2.394
Rmerge	0.106	1.599
Rmeas	0.110	1.660
Rpim	0.030	0.445
Number of reflections	17027	1718
<I/σ(I)>	19.06
Completeness [%]	99.9	99.65
Redundancy	13.3	13.7
CC(1/2)	0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292.15	Ethylene Glycol, PEG 8000, Tris, Bicin, Sodium Nitrate, Sodium Phosphate dibasic, Ammonium Sulfate