6TJ4

P. falciparum essential light chain, N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	30.237, 57.510, 86.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.860 - 1.500
R-factor	0.168
Rwork	0.167
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tj3
RMSD bond length	0.013
RMSD bond angle	1.161
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.860	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.030	0.500
Rmeas	0.040	0.570
Rpim	0.020	0.270
Number of reflections	24622	2365
<I/σ(I)>	17.68	2.06
Completeness [%]	99.0	98
Redundancy	4.2	4.2
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292.15	Tris-HCl, lithium sulfate, PEG 4000