6TJ0

Crystal structure of the bacterial cellulose secretion regulator BcsE, residues 217-523, with bound c-di-GMP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97911, 0.97927
Spacegroup name	P 41 21 2
Unit cell lengths	112.510, 112.510, 106.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.730 - 2.200
R-factor	0.207
Rwork	0.205
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.050
Phasing software	PHASER (v2.5.7)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.200	2.200
Rmeas	0.217
Rpim	0.052
Number of reflections	66468	10635
<I/σ(I)>	9.11	0.81
Completeness [%]	99.4	98.2
Redundancy	9.13
CC(1/2)	0.998	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1M 2-(N-morpholino)ethanesulfonic acid pH6, 0.2M Magnesium chloride, 4% PEG 4000, 5% Glycerol