6TIM

THE ADAPTABILITY OF THE ACTIVE SITE OF TRYPANOSOMAL TRIOSEPHOSPHATE ISOMERASE AS OBSERVED IN THE CRYSTAL STRUCTURES OF THREE DIFFERENT COMPLEXES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	112.360, 97.590, 46.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.137 *
RMSD bond length	0.014
RMSD bond angle	2.500
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.043 *
Number of reflections	18064 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	triosephosphate isomerase	4.5 (mg/ml)	0.9 M ammonium sulphate
2	1	drop	ammonium sulfate	60 (%)
3	1	drop	EDTA	1 (mM)
4	1	drop	3-(N-morpholino)-propane-sulphonic acid	0.2 (M)
5	1	drop	dithiothreitol	1 (mM)
6	1	drop	sodium azide	1 (mM)
7	1	reservoir	ammonium sulphate	60 (%)