6TI2
Structure of the Ustilago maydis chorismate mutase 1 in complex with KWL1-b from Zea mays
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.07252 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.740, 124.580, 98.210 |
Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
Resolution | 47.990 - 2.750 |
R-factor | 0.2256 |
Rwork | 0.224 |
R-free | 0.26320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fpg |
RMSD bond length | 0.006 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.820 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.087 | 0.705 |
Number of reflections | 35373 | 3546 |
<I/σ(I)> | 8.31 | 1.36 |
Completeness [%] | 98.4 | |
Redundancy | 3 | |
CC(1/2) | 0.992 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M HEPES pH 7.0, 8 % (w/v) PEG 8000 |