6THH

Crystal structure of type I-D CRISPR-Cas nuclease Cas10d in complex with the SIRV3 AcrID1 (gp02) anti-CRISPR protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	157.990, 157.990, 130.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.542 - 3.480
R-factor	0.2379
Rwork	0.236
R-free	0.25380
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.542	3.600
High resolution limit [Å]	3.480	3.480
Rmerge	0.060	0.680
Number of reflections	21713	2139
<I/σ(I)>	11.9	1.2
Completeness [%]	99.9	99.8
Redundancy	2	2
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	0.1 M Hepes 7.5, 2% (w/v) PEG 8000, 5% ethylene glycol