6THB

Receptor binding domain of the Cedar Virus attachment glycoprotein (G)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	201.460, 201.460, 112.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.241 - 2.780
R-factor	0.2025
Rwork	0.201
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vwd
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.000	2.850
High resolution limit [Å]	2.780	2.780
Rmerge	0.164	1.776
Rpim	0.037	0.412
Number of reflections	65603	4849
<I/σ(I)>	15.4	2
Completeness [%]	99.9	99.6
Redundancy	20.5	19.5
CC(1/2)	0.998	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5% (w/v) polyethylene glycol 6000, 0.1 M MES pH 6.0