6THA

Crystal structure of human sugar transporter GLUT1 (SLC2A1) in the inward conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-05
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9686
Spacegroup name	C 1 2 1
Unit cell lengths	120.000, 102.180, 69.000
Unit cell angles	90.00, 98.89, 90.00

Refinement procedure

Resolution	41.656 - 2.400
R-factor	0.2057
Rwork	0.204
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pyp
RMSD bond length	0.004
RMSD bond angle	0.650
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev-2614)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.656	41.656	2.500
High resolution limit [Å]	2.400	20.000	2.400
Rmerge	0.113	0.027	2.218
Rmeas	0.123	0.033	2.380
Total number of observations	229827
Number of reflections	32166	52	3666
<I/σ(I)>	7.2	17.59	0.72
Completeness [%]	99.9	83.9	100
Redundancy	7.145	3.135	7.611
CC(1/2)	0.998	0.998	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290.15	42-46 % polyethylene glycol 400, 100-200 mM MgCl2 and 100 mM Mops pH 7.4