6TH3
Cryo-EM structure of p62-PB1 filament (S-type)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 4 |
Software | SPRING,RELION,SerialEM,Gctf,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |