6TH1

IE1 from rat cytomegalovirus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-20
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 65 2 2
Unit cell lengths	173.049, 173.049, 134.485
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.776 - 3.400
R-factor	0.2179
Rwork	0.215
R-free	0.24420
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	HKL2Map
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.776	18.776	3.590
High resolution limit [Å]	3.380	9.950	3.380
Rmerge	0.359	0.042	5.653
Rmeas	0.361	0.042	5.688
Total number of observations	1343584
Number of reflections	16925	752	2610
<I/σ(I)>	19.08	109.79	1.02
Completeness [%]	99.0	96.7	97.2
Redundancy	79.385	65.372	80.587
CC(1/2)	1.000	1.000	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	TRIS/HCl, MgCl2