6TGX
Crystal structure of Arabidopsis thaliana NAA60 in complex with a bisubstrate analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976251 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.062, 76.165, 109.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.510 - 1.770 |
R-factor | 0.1825 |
Rwork | 0.181 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292105306 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.510 | 1.810 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.085 | 2.414 |
Rpim | 0.025 | 0.700 |
Number of reflections | 41529 | 2329 |
<I/σ(I)> | 14.9 | 1.1 |
Completeness [%] | 99.8 | 100 |
Redundancy | 12.5 | 12.7 |
CC(1/2) | 0.999 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291.15 | reservoir: 0.1 M citric acid pH 5 and 20 % (w/v) PEG 6000 protein: c=13mg/ml drop: 200nl protein solution + 200 nl precipitant |