6TER
Crystal structure of a galactokinase from Bifidobacterium infantis in complex with Galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.320, 164.929, 113.630 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 82.600 - 1.680 |
| R-factor | 0.2278 |
| Rwork | 0.224 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tep |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.853 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.600 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.108 | 0.553 |
| Number of reflections | 205822 | 10162 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 94.7 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, 0.15 - 0.25 M NaCl and 18 - 22 % (w/v) polyethylene glycol 6000) |
