6TE3

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Fe2+, Mn2+, UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 2 2 21
Unit cell lengths	96.995, 100.035, 225.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.840 - 2.300
R-factor	0.197
Rwork	0.194
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fxm
RMSD bond length	0.004
RMSD bond angle	0.618
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.840	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.118	1.476
Rpim	0.086	1.107
Number of reflections	48288	4455
<I/σ(I)>	8.4	0.9
Completeness [%]	98.7	99.7
Redundancy	4.8	4.8
CC(1/2)	0.997	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 500 mM FeCl2, 500 mM MnCl2, 1 mM UDP