6TCU
Glycogen synthase kinase-3 beta (GSK3b) in complex with ligand 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00004527943 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.783, 107.851, 104.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.650 - 2.140 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.497 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.650 | 2.390 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.035 | 0.443 |
| Number of reflections | 26305 | 7373 |
| <I/σ(I)> | 20.08 | 3.52 |
| Completeness [%] | 98.5 | 99.3 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 1.000 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18% (w/v) PEG8000 0.13 M NaCl 0.1 M Tris Acetate pH 8.0 |
