6TCI
The crystal structure of SleB N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 43.330, 43.330, 72.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.640 - 1.470 |
R-factor | 0.1738 |
Rwork | 0.170 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lpq |
RMSD bond length | 0.004 |
RMSD bond angle | 0.682 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.640 | 1.500 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.079 | 1.586 |
Rmeas | 0.079 | 0.160 |
Rpim | 0.099 | 0.231 |
Number of reflections | 12245 | 1128 |
<I/σ(I)> | 17.4 | |
Completeness [%] | 99.5 | 94.39 |
Redundancy | 8.8 | 6.7 |
CC(1/2) | 0.886 | 0.637 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350 containing 0.2 M potassium thiocyanate |