6TC6
Crystal structure of MutM from Neisseria meningitidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-03 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 66.378, 37.960, 110.303 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 42.431 - 2.900 |
R-factor | 0.2476 |
Rwork | 0.245 |
R-free | 0.29800 |
Structure solution method | MIR |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.431 | 2.952 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 19597 | 1162 |
<I/σ(I)> | 9.23 | |
Completeness [%] | 96.4 | |
Redundancy | 29.5 | |
CC(1/2) | 0.844 | 0.207 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100 mM Bicine pH 8.5; 20% (w/v) PEG 6000 |