6TBY
Phycocyanobilin-adducted PAS-GAF bidomain of Sorghum bicolor phyB
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-14 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 134.738, 134.738, 46.543 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.100 - 1.800 |
| Rwork | 0.179 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4our |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.773 |
| Data reduction software | XDS (Mar 15 2019) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.082 | 2.726 |
| Rmeas | 0.086 | 2.858 |
| Rpim | 0.026 | 0.858 |
| Number of reflections | 44840 | 2647 |
| <I/σ(I)> | 22.2 | 1.5 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 20.3 | 21.3 |
| CC(1/2) | 1.000 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 283.15 | (100 mM Tris/HCl pH 8.5, 500 mM NaCl, 9% w/v PEG4000 |
