6TBR
Glycosylated AA13 Lytic polysaccharide monooxygenase from Aspergillus oryzae in P1 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.04 |
Spacegroup name | P 1 |
Unit cell lengths | 46.060, 49.760, 57.100 |
Unit cell angles | 109.37, 90.07, 95.46 |
Refinement procedure
Resolution | 53.840 - 1.700 |
R-factor | 0.1945 |
Rwork | 0.192 |
R-free | 0.24640 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.019 |
RMSD bond angle | 1.853 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.840 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmeas | 0.077 | 0.455 |
Number of reflections | 48576 | 3521 |
<I/σ(I)> | 2.87 | 9.04 |
Completeness [%] | 92.6 | |
Redundancy | 1.68 | |
CC(1/2) | 0.994 | 0.877 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.01 M ZnCl2, 14% PEG6000, 0.1 M MES pH 6.0, 30% glycerol |