6TBN

Crystal structure of CIAO1-CIAO2B CIA core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	I 4
Unit cell lengths	125.580, 125.580, 85.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.860 - 2.000
R-factor	0.1819
Rwork	0.180
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fm0
RMSD bond length	0.006
RMSD bond angle	0.704
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	44669	4240
<I/σ(I)>	14.8	0.72
Completeness [%]	99.4
Redundancy	53.9
CC(1/2)	0.998	0.142

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8.5, 25% PEG MME 2k