6TAF

Bd0314 DslA E154Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97622
Spacegroup name	P 21 21 2
Unit cell lengths	84.729, 47.854, 49.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.308 - 1.336
R-factor	0.178
Rwork	0.177
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	mutant protein
RMSD bond length	0.005
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.380
High resolution limit [Å]	1.336	1.370
Number of reflections	45432	2267
<I/σ(I)>	12.2
Completeness [%]	98.4
Redundancy	12.5
CC(1/2)	0.994	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.2M lithium sulphate 0.1M sodium citrate pH 5.5 20% PEG 3000