6TAA
STRUCTURE AND MOLECULAR MODEL REFINEMENT OF ASPERGILLUS ORYZAE (TAKA) ALPHA-AMYLASE: AN APPLICATION OF THE SIMULATED-ANNEALING METHOD
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.040, 67.183, 133.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.700 - 2.100 |
| R-factor | 0.198 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.063 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 * |
| Rmerge | 0.048 * |
| Total number of observations | 66210 * |
| Number of reflections | 23794 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | PEG8000 | 8 (%(w/v)) | |
| 5 | 1 | resrvoir | PEG8000 | 16 (%) | |
| 6 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 7 | 1 | reservoir | 2 (mM) |
