6TA7
CRYSTAL STRUCTURE OF HUMAN G3BP1-NTF2 IN COMPLEX WITH HUMAN CAPRIN1-DERIVED SOLOMON MOTIF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-05-13 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.558, 100.038, 113.345 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.540 - 1.930 |
R-factor | 0.2081 |
Rwork | 0.207 |
R-free | 0.25180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fw5 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.777 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.540 | 1.997 |
High resolution limit [Å] | 1.928 | 1.928 |
Number of reflections | 66520 | 4630 |
<I/σ(I)> | 14.62 | 0.68 |
Completeness [%] | 94.3 | 66.43 |
Redundancy | 13.5 | |
CC(1/2) | 0.999 | 0.320 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium chloride, 0.1M Tris 8.0, 20% w/v PEG 4000 of the Proplex crystallization screen (Molecular Dimensions) |