6T9Q
Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 65 |
| Unit cell lengths | 55.407, 55.407, 41.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.460 - 1.150 |
| R-factor | 0.1294 |
| Rwork | 0.128 |
| R-free | 0.15470 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.964 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.460 | 1.191 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.138 | 0.487 |
| Rmeas | 0.154 | 0.650 |
| Rpim | 0.069 | 0.427 |
| Number of reflections | 25072 | 1920 |
| <I/σ(I)> | 6.82 | 1.36 |
| Completeness [%] | 96.3 | 74.84 |
| Redundancy | 4.2 | 1.6 |
| CC(1/2) | 0.975 | 0.464 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 200mM Sodium formate 18% PEG3350 |
