6T9G
CRYSTAL STRUCTURE OF AN ENDOGLUCANASE D213A FROM PENICILLIUM VERRUCULOSUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2019-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.437, 89.965, 91.587 |
Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
Resolution | 44.983 - 2.296 |
R-factor | 0.213151746088 |
Rwork | 0.211 |
R-free | 0.24807 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i6s |
RMSD bond length | 0.008 |
RMSD bond angle | 0.965 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.983 | 2.430 |
High resolution limit [Å] | 2.296 | 2.296 |
Number of reflections | 56494 | 7727 |
<I/σ(I)> | 9.51 | |
Completeness [%] | 96.6 | |
Redundancy | 6.41 | |
CC(1/2) | 0.997 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 285 | PEG 3350,bis-tris pH 5,5, magnesium chloride |