6T9F
CRYSTAL STRUCTURE OF EN ENDOGLUCANASE S308P FROM PENICILLIUM VERRUCULOSUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2019-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.358, 77.391, 90.923 |
| Unit cell angles | 81.81, 76.69, 75.86 |
Refinement procedure
| Resolution | 59.750 - 2.248 |
| R-factor | 0.219809562325 |
| Rwork | 0.218 |
| R-free | 0.25601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i6s |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.750 | 2.380 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Number of reflections | 198022 | 8958 |
| <I/σ(I)> | 5.93 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.992 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | sodium formate, PIPES pH 7.0, ammonium acetate |
