6T8E
Crystal structure of native xylose isomerase from Piromyces E2 grown in yeast, in complex with xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.454, 79.267, 91.878 |
| Unit cell angles | 115.46, 89.99, 117.07 |
Refinement procedure
| Resolution | 46.600 - 1.860 |
| R-factor | 0.1405 |
| Rwork | 0.139 |
| R-free | 0.17630 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5nh5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | REFMAC (5.8.0238) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.066 | 0.380 |
| Rmeas | 0.090 | 0.518 |
| Rpim | 0.060 | 0.350 |
| Number of reflections | 135735 | 5669 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 93.6 | 79.1 |
| Redundancy | 2.1 | 1.9 |
| CC(1/2) | 0.931 | 0.286 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 14-17 % PEG3350, HEPES, pH 7 |
