6T7O
X-ray structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)
Replaces: 5OOXExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.855, 45.855, 117.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.165 - 1.600 |
| R-factor | 0.162230671118 |
| Rwork | 0.161 |
| R-free | 0.18193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.143 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.165 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.632 |
| Number of reflections | 17357 | 1692 |
| <I/σ(I)> | 28.12 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 13.1 | |
| CC(1/2) | 0.999 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15M Citric acid pH 4.0, 24% PEG 6000 |
