6T7G

Bacteroides salyersiae GH164 beta-mannosidase in complex with mannoimidazole

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.976230
Spacegroup name	P 1
Unit cell lengths	69.710, 104.743, 170.568
Unit cell angles	92.29, 97.27, 106.30

Refinement procedure

Resolution	168.660 - 1.800
Rwork	0.206
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6t5o
RMSD bond length	0.008
RMSD bond angle	1.427
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	168.660	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	424506	21158
<I/σ(I)>	6.9
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.998	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium tartrate, PEG 3350